TALENs Service Details

TALEN Construction 1-2 months
Transgenic expression vectors can be constructed based on individual requirements, and on available sequences and base constructs.

  • Design proposal for a custom TALEN heterodimer cleaving the customer’s target
  • Synthesis and sequence verification of TALEN expression plasmids
  • Synthesis of TALEN mRNA
Pronucleus Microinjection of TALEN 2 months
  • Co-injection of verified TALEN mRNA and template in C57Bl/6 zygotes
  • Transfer of injected zygotes into foster mice
  • Genotyping and Sequencing of offspring to identify correctly modified target regions

Dependent on the mouse strain and vector, a minimum number of offspring and founder is guaranteed.

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