CRISPR/Cas9 Service Details

CRISPR design and synthesis 3-4 weeks
For each target two or three different guide RNAs (sgRNA) sequences will be identified.

  • Design proposal for 2-3 custom sgRNAs, targeting the gene or region of interest
  • Analysis of potential off-targets
  • Synthesis of sgRNA
  • For knock-in models: design and synthesis of template DNA; either a large oligo nucleotide or small targeting vector containing the desired knock-in
Pronucleus Microinjection of CRISPR/Cas9 2 months
  • Co-injection of sgRNAs, Cas9 protein and template DNA in C57Bl/6 zygotes
  • Transfer of injected zygotes into foster mice
  • Genotyping and Sequencing of offspring to identify correctly modified target regions

Depending on the specificity of the selected sgRNA sequences, a thorough analysis of off-target effects is recommended. Please inquire for more details.

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