The transgenic targeting procedures developed at PolyGene allow the introduction of expression cassettes at specific and well-defined loci. This solution is most appropriate for high throughput target evaluation, since it warrants predictable and reproducible transgene expression patterns.
Transgenic Targeting is perfectly suited for large-scale in-vivo projects as well as for mutation screenings and comparisons of disease alleles. Accordingly, this technology is ideal to further the functional genomics approach by in-vivo target validation.
Benefits
Overall, the PolyGene Transgenic Targeting technology saves considerable time and money, which is otherwise spent on analyzing multiple unnecessary transgenic lines resulting from random transgene integrations.
PolyGene’s proven concept for mouse model development offers unique advantages
A single mouse line is needed for any one transgene. Optimized procedures allow for swift generation of stable transgenic mice. Transgenes are introduced in mouse lines with pre-characterized gene expression patterns.
Insertional effects can be excluded by comparison with the marker-gene integration control mouse.
Additional new mutations are easily introduced, thus facilitating high throughputscreening.
Experiment evaluations and comparisons of different transgenic mouse lines are more meaningful.
High procedural reliability inspires development of inducible and tissue specifictransgene expression models.
PolyGene is committed to widening its range of proprietary mouse models, and welcomes collaboration opportunities with academic and industrial researchers. Please contact us.
Target validation, new perspectives
Transgenic Targeting and in vitro ES cell differentiation allow reliable selection and validation of but the most promising drug target candidates.
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