Transgenic animal models are the workhorse of mammalian genetics. Innovative as well as standard methods allow for the production of animals carrying foreign genes in a predefined manner: as tissue-specific genes, as single-copy or multi-copy cluster, as fusion genes, or in inducible or repressible form.
PolyGene is offering services for the development of comprehensive projects, as well as milestone steps for partial services (e.g., microinjection services, or vector construction).
Designed gene(s) or BACs are injected into the pronucleus of the oocyte and integrate randomly into the genome. Our microinjection services are available for a variety of mouse strains, including C57BL/6, FVB, or CB6F2 mice as well as Sprague Dawley rats.
The main advantage of this technique is the short development time. The complete development of a transgenic mouse line takes about 6 months. Level and pattern of the transgene expression can vary due to unknown copy number and integration site, which makes it necessary to analyze several independent transgenic lines.
Please note also our guarantees for number of offspring and founders.