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Transgenic Mouse or Rat Project

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Gene cloning (in 2 months, or less)
- construct the probe, screen the library and isolate clones (genomic BAC or cDNA), or retrieve appropriate sequences from public libraries
- verify clone inserts by Southern and/or PCR technology and appropriate sequencing
Desired origin:
Mouse Rat Other Species
Other Species(specify):
Is the full sequence known/published:
Yes Don’t know No
Type of sequence:
cDNA Genomic
Remarks:

Vector construction (in 5-6 months, or less)
Expression Vector
Promoter, transgene (cDNA) and regulatory elements (e.g. NLS, introns or poly-A)

some fragments to be isolated by
all sequences available PolyGene
include inducible transgenic strategie
For complex vectors, please contact either:
stefan.selbert@polygene.ch Tel. (direct line) +41 44 828 63 83 or
doron.shmerling@polygene.ch Tel. (direct line) +41 44 828 63 82
Screening strategy and PCR analysis by:
PolyGene Yourself
Remarks:

Pronucleus injection (in 2-3 months or less)
- Injection of up to 3 x 150 mouse oocytes (e.g. C57BL/6, FVB, or CB6F2)
- Injection of up to 2 x 200 Sprague Dawley oocytes
- Transgenesis in other species is available on request
- Transfer into foster mice, progeny mating, tail biopsy of the offspring and genotyping
Desired oocyte strain:
Mouse:
C57BL/6 FVB/N CB6F2
Rat:
Sprague Dawley
Other strains or species (specify):
Screening strategy and PCR analysis by:
PolyGene Yourself
Remarks:









PolyGene AG
Riedmattstrasse 9
CH-8153 Rümlang
Phone: +41 44 828 63 80
Fax: +41 44 828 63 81