Through extensive involvement in genetic engineering and the generation of transgenic animals, the PolyGene team of scientists has an excellent academic and industrial track record.
Beyond outstanding microinjection skills and proficiency in harnessing an extensive range of molecular- and cell biology techniques, particularly noteworthy savoir-faire at PolyGene comprises:
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design and evaluation of animal models, drug testing and response evaluation in mice, realization of behavioural studies, neuroanatomical and neuropathological assessment, complemented by broad knowledge in mammary gland, heart and skeletal muscle biology, as well as in immunology. |
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Expertise with Cre/lox systems (in-vitro & in-vivo) and the use of various mutant lox sites in Recombinase Mediated Cassette Exchange (RMCE) procedures, as well as practical experience with Flp/FRT, |
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use of innovative selection markers (e.g. DipA) and regulatory sequences such as LoxP, FRT, IRES, SMAR, conditional stop, … for influencing gene expression, |
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sub-cellular fractionation, immunohistochemistry, immunoprecipitation, affinity purification, immunofluorescence and apoptosis assays (TUNEL) in the context of gene expression analysis, |
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utilization of different expression markers (including PLAP and C-, Y- & GFP), |
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exploitation of siRNA and antisense RNA technology, |
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in-vitro differentiation of ES-cells into embryoid bodies and further, into a variety of cell types, |
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establishment and transfection of primary cell lines from organs (e.g. splenocytes, neuroblasts) of genetically modified mice. |
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