One step closer to understanding T-cell leukemia

T-cell leukemia is rare blood cancer in the own immune system. T-cell acute lymphoblastic leukemia, a special form of T-cell leukemia, was investigated by Belgian researchers with striking findings.

In a recently conducted patient study, a R98S mutation in ribosomal protein L10 recurred in 8% of children with T-cell acute lymphoblastic leukemia.

This protein plays an important role in the JAK-STAT signaling pathway which transmits chemical signals from the extracellular matrix into the nucleus and finally influences the transcription of DNA and expression of genes.

JAK-STAT signaling pathway
JAK-STAT signaling pathway.
(By Peter Znamenkiy – Own work, Public Domain, https://commons.wikimedia.org/w/index.php?curid=912703)
The three main components in the JAK-STAT pathway are the cell surface receptor (JAnus Kinase) and two Signal Transducer and Activator of Transcription (STAT) proteins.

The group of Kim de Keersmaecker from KU Leuven in Belgium investigated this mutation in greater detail by creating a customized gene knock-in mouse model with PolyGene.

In the transgenic R98S mouse, several proteins in the oncogenic JAK-STAT pathway are overexpressed, and increased sensitivity to clinically used JAK-STAT inhibitors like pimozide was observed. These findings link a ribosomal mutation to the JAK-STAT pathway, adding to the rapidly expanding repertoire of oncogenic mechanisms exploited by ribosomal defects in cancer. Moreover, the JAK-STAT cascade was identified as an attractive therapeutic target in RPL10 R98S positive T-cell acute lymphoblastic leukemia.

Interested in knock-in services for your gene(s) of interest? PolyGene offers:

>> Online Publication

Novel humanized mouse model for heart disease research

Sudden cardiac death is caused by arrhythmias, which is defined as any deviation of the heart beat. Electrical heart disease leading to arrhythmias represents a major public health issue.

The histidine-rich calcium-binding protein (HRC) Ser96Ala variant is known as potential biomarker for sudden cardiac death in patients with a heart muscle disease. HRC is a regulator of sarcoplasmic reticulum (SR) Ca2+ uptake, storage and release. The SR is a membrane bound structure found within muscle cells.

An HRC-Ala96 mouse model using transgenic techniques was developed previously. However, transgenesis has limitations: random positioning, unknown copy number and dependent expression of the inserted DNA. Other issues in the HRC-Ala96 transgenic model arose at the developmental expression of the desired gene dictated by the a-MHC-promoter and the heterologous human gene expression.
To overcome the limitations of HRC overexpression, PolyGene employed a gene targeting knock-in strategy and generated a mouse model carrying an HRC mutation at position 81 (serine 81 to alanine), the homologous region of the human HRC (serine 96 to alanine). This humanized mouse model and subsequent pathophysiological analyses were now published.
(Adapted from Tzimas et al. 2017)

HRC-Ser96Ala_Knock-in_Strategy PolyGene
Generation of the HRC-Ser81Ala knock-in mouse model.
Schematic representation of the WT and targeted (TG) HRC-Ser81Ala allele. The targeting vector contains two arms for homologous recombination, separated by a FRT flanked neomycin cassette. The Ser81Ala point mutation is introduced in exon 1 of HRC. Mating of the targeted mice with FLP deleter mice removes the neomycin cassette and results in the generation of targeted and neo-deleted mice.

The generated mutant HRC mouse is physiologically very relevant to the human condition, since the murine endogenous HRC gene remains intact except the specific Ser81Ala mutation. It showed increased mortality in the absence of structural or histological abnormalities, indicating that early death may be arrhythmia-related.
This strongly suggests that indeed Ser96Ala in humans is associated with depressed cardiac function and possible cardiac-related sudden death.

This research was funded by EUTrigTreat, an international consortia founded by the EU, to address both clinical and basic sciences questions about dangerous arrhythmias by translational and interdisciplinary strategies.

>> Online Publication

LRP4 mouse model helps to understand rare skeletal disease

Sclerosteosis is a rare autosomal recessive bone disorder marked by excessive bone growth of the skull and tubular bones. Up to now, research showed: sclerosteosis was caused by loss-of-function mutations in the SOST gene, encoding sclerostin.

Recently, researchers within the european SYBIL consortium identified disease causing mutations in LRP4, a binding partner of sclerostin, in three Sclerosteosis patients. Upon binding to sclerostin, LRP4 can inhibit the canonical WNT signaling which is known to be an important pathway in the regulation of bone formation.
A Lrp4 mutated sclerosteosis knock-in mouse model generated by PolyGene showed to be a good model for the human sclerosteosis phenotype. This model will be used for further investigation of the LRP4 regulating bone formation mechanism.

This work was published in the Journal of Bone and Mineral Research on May 6th 2017 by the group of Prof. Wim van Val located at the Center of Medical Genetics at the University of Antwerp in Belgium.

>> Online Publication

Inspiring ECTS meeting and PolyGene seminar continued

Last tuesday ended the 44th European Calcified Tissue Society Congress (ECTS) in Salzburg, Austria and PolyGene looks back on vivid talks and fruitful networking.

Every year, ECTS creates a platform for researchers and clinicians working in the musculoskeletal field to strengthen collaborations and discuss the latest advances.
PolyGene brings great expertise in developing relevant in vivo models of rare skeletal and common skeletal diseases to many european research institutes (e.g. within the EU-funded SYBIL program).

The next PolyGene Seminar will take place June 1th at the Swiss institute for regenerative medicine (SIRM) in Taverne – Torricella, Switzerland.

Dr. Doron Shmerling, CEO of PolyGene and research scientist Dr. Péter Horváth present our latest in-house research on inducible gene expression systems, as well as genetic modifications using CRISPR/Cas9 and similar technologies.
Get more information on our event calendar, we are looking forward meeting you soon.

Special PolyGene seminar at University of Zurich

Join PolyGene for a special seminar on „Innovative Genetic Technologies in Transgenic Model Generation“. The event takes place April 19th 2017, 5pm, University of Zurich Y23 G04. The first talk will focus on CRISPR and transgenic rodent model services presented by Stephan Sonntag. In the second talk, Péter Horváth will give insight in state-of-the-art inducible gene expression systems and PolyGene’s in-house research. We are looking forward to meeting you there.

We look back on a successful LS2 Meeting at University of Zürich

Lets start with a quote from Didier Picard from University of Geneva and Chair of the LS2 Annual Meeting 2017 in Zürich:

If you are a life scientist in Switzerland, this is the place to be and to be seen, at least once a year. Its dense program is designed to appeal to everyone, to give everyone the opportunity, again at least once a year, to get exposed to great science in their own field, but also to exciting and thought – provoking stuff from different fields or from the interfaces between science and the real world. The LS2 meeting is a great opportunity to network, to meet your old buddies from your undergraduate days, graduate school, and committees you have served on, your former professors, many of your suppliers, your current and future collaborators, and perhaps your future boss.

We had a great time there, meeting indeed old friends and customers but also created new bonds to possible future collaborations.
Our CEO Doron Shmerling has been an invited speaker within the Session “Start a start-up”. He brought his longlasting experience in managing a small enterprise to more than 150 students.

Life Science Switzerland LS2 Meeting in Zurich

Connect with us at the LS2 Meeting at the University of Zurich 2nd-3th February, 2017.

LS2 is the annual meeting of Swiss experts in a broad range of life science disciplines.
Discover the latest, most exciting findings in the field, from Molecular and Cellular Biosciences to Proteomics and Physiology, or enjoy the Satellite Workshop on opportunities in academic and non-academic careers.

We invite our clients and researchers interested in transgenic animal model research to visit us at booth no.3!

Our CEO Doron Shmerling will also participate at an exciting panel discussion about starting a start-up. This event takes place Friday, 10:30 – 12:30 during the Parallel Symposia III.

More information can be found directly on the LS2 webpage: LS2 Annual Meeting.
Wondering where you can meet PolyGene next? Check out our event calendar.

December Offerings

Late December Offering:

Free genotyping and breeding of your new PolyGene mouse line (including CRISPR/Cas9 gene targeting, traditional ES cell targeting and pronuclear microinjection of your DNA), when ordering a complete mouse model!

  • order before 30th December 2016.
  • add the code “FreeGenotyping” in your quote.

Mid December Offering:

Get sperm freezing of your new mouse line for free, if you order a complete mouse model.

  • order before 30th December 2016.
  • add the code “FreeFreezing” in your quote.

Early December Offering:

Get free shipment of your next mouse model.

  • order before 30th December 2016.
  • add the code “FreeShipment” in your quote.
The offers mentioned above can not be combined.
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